Microinjection
Microinjection (pronunciation: /ˌmaɪkroʊɪnˈdʒɛkʃən/) is a method of introducing substances into cells by means of a fine needle, used especially in genetic research.
Etymology
The term "microinjection" is derived from the Greek words "micros" meaning small and "enienai" meaning to send into.
Definition
Microinjection is a technique used in genetic engineering to insert genetic material into a single cell. The process involves using a glass micropipette to deliver the material into the cell's cytoplasm or nucleus. This method is often used in molecular biology, biochemistry, and genetics research.
Procedure
The microinjection procedure involves several steps. First, the cell is immobilized, often using a holding pipette. Then, a microinjector is used to pierce the cell membrane and deliver the substance into the cell. The substance can be a variety of things, including DNA, RNA, or proteins.
Applications
Microinjection has a wide range of applications in various fields of research. It is commonly used in genetic engineering to introduce new genes into cells. In reproductive biology, it is used in procedures such as Intracytoplasmic sperm injection (ICSI) where a single sperm is injected directly into an egg. In neuroscience, it is used to introduce substances into neurons for study.
Related Terms
- Genetic engineering
- Molecular biology
- Biochemistry
- Genetics
- Intracytoplasmic sperm injection (ICSI)
- Neuroscience
External links
- Medical encyclopedia article on Microinjection
- Wikipedia's article - Microinjection
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