HPS stain
HPS Stain
HPS stain (pronounced as H-P-S stain), also known as Hematoxylin Phloxine Saffron stain, is a type of histological stain used in pathology to examine tissue samples under a microscope.
Etymology
The term "HPS stain" is an acronym derived from the names of the three dyes used in the staining process: Hematoxylin, Phloxine, and Saffron.
Usage
HPS stain is commonly used in histopathology to differentiate between various types of cells and tissues. It is particularly useful in identifying and studying the structure of connective tissues, epithelial tissues, and muscular tissues.
Procedure
The HPS staining procedure involves the sequential application of hematoxylin, phloxine, and saffron dyes to a tissue sample. Hematoxylin stains the cell nuclei blue, phloxine stains the cytoplasm and extracellular matrix pink, and saffron provides a yellow counterstain.
Related Terms
- Histology: The study of the microscopic structure of tissues.
- Staining: The process of coloring the microorganisms with the help of a dye that is not readily decolorized by alcohol or water.
- Hematoxylin: A basic dye used in histology and cytology to stain cell nuclei blue.
- Phloxine: A synthetic dye used for staining in histology.
- Saffron: A natural dye used in histology for its yellow color.
External links
- Medical encyclopedia article on HPS stain
- Wikipedia's article - HPS stain
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