Coomassie brilliant blue
Coomassie Brilliant Blue
Coomassie Brilliant Blue (pronunciation: /kuːˈmæsi ˈbrɪljənt ˈbluː/) is a synthetic dye used predominantly in biochemistry for the staining of proteins in polyacrylamide gel electrophoresis, as well as in the Bradford protein assay.
Etymology
The term "Coomassie" is derived from the name of the African kingdom of Kumasi, which was a British protectorate in the late 19th century when the dye was first produced. "Brilliant Blue" refers to the intense blue color of the dye.
Usage
Coomassie Brilliant Blue is available in two forms: G-250 and R-250. The G-250 variant is used in the Bradford protein assay, while the R-250 variant is used for staining proteins in polyacrylamide gels. The dye binds to proteins, allowing them to be visualized and quantified.
Related Terms
- Biochemistry: The study of chemical processes within and relating to living organisms.
- Polyacrylamide gel electrophoresis: A method used in biochemistry, molecular biology, genetics, and clinical chemistry to separate biological macromolecules, usually proteins or nucleic acids, according to their electrophoretic mobility.
- Bradford protein assay: A spectroscopic analytical procedure used to measure the concentration of protein in a solution.
- Kumasi: A city in Ashanti Region, and is among the largest metropolitan areas in Ghana.
External links
- Medical encyclopedia article on Coomassie brilliant blue
- Wikipedia's article - Coomassie brilliant blue
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